2025-06-102025-06-102023-12-15PAMPOLHA, Ana Beatriz Oliveira. Competência vetorial do mosquito Culex quinquefasciatus (SAY, 1823) ao Orthoflavivirus ilheusense (ILHV). Orientador: Joaquim Pinto Nunes Neto.; Coorientadora: Jacqueline Cortinhas Monteiro. 2023. 45 f. Trabalho de Curso (Licenciatura em Ciências Biológicas) – Faculdade de Ciências Biológicas, Universidade Federal do Pará, Belém, 2025. Disponível em: https://bdm.ufpa.br/handle/prefix/8306. Acesso em:.https://bdm.ufpa.br/handle/prefix/8306With the increase in deforestation and constant climate change, the proximity of wild animals to humans is inevitable and the risks of the emergence of zoonoses arise through contact between species, whether direct or indirect, through vectors. Orthoflavivirus ilheusense (ILHV) is an emerging arbovirus of the Flaviviridae family with a primarily avian cycle, detected in different genera of mosquitoes and already registered in humans in several countries, causing infection with febrile symptoms and encephalitis. However, for transmission to be possible the virus depends, first and foremost, on the mosquito vector capacity, which varies according to its immunological response because, due to exposure to pathogens through the practice of hematophagy, it has physiological barriers that hinder their susceptibility to infection. Therefore, it is essential to determine the vector competence of Culex quinquefasciatus for transmitting ILHV, as it is a common mosquito in Brazilian homes associated with the transmission of originally avian arboviruses that cause encephalitis. To carry out the study, two infections were carried out, via oral infection, with 4 mL of goose blood and viral suspension obtained from viral stock with a 1:1 ratio, with a control group of uninfected females of the same generation. Segmentation was carried out on the 7th, 14th and 21st day post-infection (DPI) separated into saliva, body and head and then viral isolation was performed for subsequent analysis in the indirect immunofluorescence test (IIF) of the samples. Viral titer results were obtained using samples diluted in a 10-fold series, as well as calculating infection, dissemination and transmission rates. A total of 70 mosquitoes were analyzed individually, resulting from the two infections. Through the IIF test, the absence of the presence of the virus was indicated for samples from each DPI, with a 0% infection, dissemination, and transmission rate. Therefore, for the methodology used, Culex quinquefasciatus was refractory to the virus, that is, it is not a vector susceptible to infection and transmission of Orthoflavivirus ilheusense. Furthermore, it is necessary to carry out more research to study the mosquito's immunological response to the virus and the mechanisms of action of ILHV on mosquito infection barriers to better understand its vectorial capacity.Acesso AbertoArbovírusFlaviviridaeCulexInfecções por flaviviridaeFlavivirusInfecções por arbovírusVetores de doençasMosquitos vetoresDoenças transmitidas por mosquitosCNPQ::CIENCIAS BIOLOGICAS::BIOLOGIA GERALTrabalho de Curso - Graduação - MonografiaAttribution-NonCommercial-NoDerivs 3.0 Brazil