2019-04-122019-04-122017MACIEL, Yasmin Cristina Costa. Avaliação da taxa de formação de blastocistos após a injeção intracitoplasmática de espermatozoide em oócitos em metáfase I de mulheres submetidas à fertilização in vitro. Orientador: João Paolo Bilibio. 2017. 52 f. Trabalho de Curso (Bacharelado em Medicina) - Faculdade de Medicina, Instituto de Ciências da Saúde, Universidade Federal do Pará, Belém, 2017. Disponível em: http://bdm.ufpa.br/jspui/handle/prefix/1274. Acesso em:.http://bdm.ufpa.br/jspui/handle/prefix/1274In vitro fertilization (IVF) is a procedure performed to overcome female and male infertility. During oocyte aspiration, it can occur retrieval of mature oocytes, in metaphase II (MII), or meiotically immature, in metaphase I (MI). These can spontaneously mature in vitro for MII within a few hours. As women age, there is a decrease in the quantity and quality of oocytes collected. The increase in pregnancy rate is directly related to the number of blastocysts obtained in the IVF cycle. Objective: To evaluate blastocysts formation rates from MI oocytes. Methods: Cross-sectional study. A total of 164 patients were selected to perform IVF at the Pronatus Clinic from January 2013 to December 2016. Results: In total, 1467 oocytes were fertilized by ICSI, which 1153 were in the MII stage and 314 in the MI. Considering these 314 MI, 201 matured in vitro for MII (MI-MII) and 113 remained in the MI stage after 3 hours in culture. The mean age of the patients was 35.6 years. In vitro maturation rate was 64.01%. Fertilization rate in MI oocytes was the lowest (31.9%), while MII oocytes had the best rate (79.1%). The comparison of fertilization rate showed that MI oocytes have 88% lower chance of forming embryo with 2 pronuclei than MII oocytes (p<0.001). It was noted that embryos from MI stage have the highest incidence of multinucleation and are 2.63 times more risk to have abnormalities of cell division in relation to MII (p=0.008). The blastocyst formation rate was higher in embryos from MII oocytes (36.4%), MI-MII oocytes rate was 11.4% and embryos from MI oocytes was 0.8%. The risk of MI oocytes not forming a blastocyst is 99% in relation to the MII (p<0.001). When correlating age with both oocyte retrieval and blastocyst formation, it was observed that the quantity of MII oocytes decreased with increasing age (p=0.002) and there was a decrease in blastocysts formation from these oocytes in patients over 40 years (p=0.03). Conclusion: The use of MI oocytes in IVF should be discouraged, since they have low potential for embryonic development and form embryos of low quality, with a rate of blastocyst formation of less than 1%. However, the use of in vitro matured MI for MII oocytes can be stimulated in IVF cycles, as an alternative to low obtaining MII oocytes in elderly women.Acesso AbertoBlastocistosFertilização in vitroMetáfase IMulherCNPQ::CIENCIAS DA SAUDE::MEDICINA::CLINICA MEDICA::GINECOLOGIA E OBSTETRICIATrabalho de Curso - Graduação - Monografia