2020-01-102020-01-102016SILVA, Tamyris Pegado de Souza e. Análise de biomarcadores bioquímicos em peixes provenientes de regiões com histórico de contaminação por arsênio na foz do rio Amazonas. Orientadora: Lílian Lund Amado. 2016. 53 f. Trabalho de Curso (Bacharelado em Oceanografia) - Faculdade de Oceanografia, Instituto de Geociências, Universidade Federal do Pará, Belém, 2016. Disponível em: https://bdm.ufpa.br/jspui/handle/prefix/2724. Acesso em:.https://bdm.ufpa.br/jspui/handle/prefix/2724Estuaries are important components of the coastal zone and the pollution of it is considered a critical environmental problem. The Porto de Santana/AP is located on the banks of the Amazon River and was the arrival point of manganese ore mined in Serra do Navio/AP. The ore tailings were disposed in an artificial lagoon releasing arsenic into the environment. This metalloid can be a reactive oxygen species generator and generate oxidative stress in organisms exposed to it. Biomarkers can be useful in determining the measure of impact on the health of the biota. The aim of this study was to evaluate and relate the analysis of biochemical biomarkers in fish liver of the species Propimelodus eigenmanni with the speciation of arsenic in the water. Four samples were taken: rainy 1, dry season 1, dry season 2 and rainy 2 in two rivers with anthropogenic contamination history for arsenic, located in Amapá (Beija-flor and Amazon) and a river in Pará (Campumpema) with no history of direct contamination by this metalloid. Fish were collected through angling, killed by marrow section, biometrics was made (weight, standard length and length) and the liver was dissected and stored in a freezer at -80°C for biomarker measurements. The abiotic variables were measured using a multiparameter device and water for dosage of inorganic species of arsenic was collected in Falcon’s tubes and stored at 4 °C. In addition to the speciation of arsenic analysis in water were analyzed in fish two biomarkers of exposure, glutathione-S-transferase (GST) and total antioxidant capacity against peroxyl radicals (ACAP) and a biomarker of effect, lipid peroxidation (LPO). Statistical analyzes were executed through ANOVA two-way, followed by post-hoc test (Tukey) and the significance level was 5%. In the case of biometrics was held nonparametric Kruskal-Wallis test. The arsenate was the only form of inorganic arsenic found in water. The arsenate concentrations were higher in Beija-flor and Amazon rivers and lower in Campumpema river. The GST activity and LPO did not differ between P. eigenmanni from Campumpema and Beija-flor rivers and between the sampling periods. The absence of induction of detoxifying enzyme can be related to the fact that there is a possibility of gills metabolize pollutants before they reach the liver, while chronic exposure may explain the lack of change in levels of LPO. At rainy 1, in the Amazon organisms, GST activity showed an increase being accompanied by the decrease in LPO, probably due to the higher value of arsenate in this river during the period in question. The fish showed no difference in ACAP, leading to infer that the amount of arsenic to which the organisms are exposed is not enough to induce their total antioxidant capacity. Although arsenic being in one of its bioavailable and toxic forms, in most of the analyzed periods, the redox balance of organisms is not being changed by xenobiotics in the environment, including arsenate.Acesso AbertoBagre (Peixe) - Amazonas, RioArsênio - toxicologia - Amazonas, RioCNPQ::CIENCIAS EXATAS E DA TERRA::OCEANOGRAFIA::OCEANOGRAFIA BIOLOGICATrabalho de Curso - Graduação - Monografia