2023-03-092023-03-092022ROCHA, Cammyla Souza de Sá; YOSHINO, Nilson Shinichi. Análise de lavados peritoneais pela citometria de fluxo de pacientes com adenocarcinoma gástrico. Orientador: Geraldo Ishak. 2022. 37 f. Trabalho de Curso (Bacharelado em Medicina) - Faculdade de Medicina, Instituto de Ciências da Saúde, Universidade Federal do Pará, Belém, 2022. Disponível em: https://bdm.ufpa.br:8443/jspui/handle/prefix/5389. Acesso em:.https://bdm.ufpa.br/handle/prefix/5389Background: Gastric cancer is one of the main tumors in actuality, being the 4th most common in the world. The treatments for such disease vary according to classification and degree of disease. Peritoneal metastasis, which is peritoneal cavity dissemination of free tumor cells, can often occur. The diagnosis and treatment of this degree of adenocarcinoma is still being discussed in order to find more reproducible and reliable methods. This paper analyzes flow cytometry as an alternative for diagnostics and extensive peritoneal lavage as treatment. Methods: A counting of free tumor cells via flow cytometry was made in each moment of the gastrectomy in the 3 steps of peritoneal lavage, which are the following: 1 liter of physiological saline was used at the moment of opening of the abdominal cavity (1st lavage); 1 liter right after resection of the tumor (2nd lavage) and 9 liters after changing sterile gloves (3rd lavage). In each step, 20 mL were withdrawn from the lavage and analyzed through flow cytometry, with staining of fluorochromes CD326 and CD45 and then the Tumor Cells/Leukocytes Ratio - TLR was calculated. Results: An intermediate TLR was obtained in the 1st lavage, a higher TLR on the 2nd and a lower TLR on the 3rd, with statistically significant difference between groups. Non parametric tests were used for statistical analysis (Kruskall-Wallis test and Student-Newman-Keuls comparison test). Conclusion: We have concluded that: 1 - there were metastatic precursors in the peritoneal cavity before tissue mobilization in all patients; 2 - after the tumor resection surgery, there was an expressively larger number of metastatic precursors in the cavity; 3 - with extensive peritoneal lavage there was a smaller quantity of free tumor cells compared to before the surgical procedure, promoting an expressive decrease on the registers of such cell lineage comparing to the 2nd lavage, possibly contributing to reduced chances of peritoneal metastization and a better quality of life and clinical outcomes.Acesso AbertoCâncer gástricoCitometria de fluxoCarcinomatose peritonealLavado peritonealGastric cancerFlow cytometryPeritoneal metastasisExtensive peritoneal lavageCNPQ::CIENCIAS DA SAUDE::MEDICINA::CLINICA MEDICA::CANCEROLOGIATrabalho de Curso - Graduação - Monografia