2022-08-022022-08-022022-04-27LOPES, Emilly Monteiro. Seleção de bactéria probiótica da espécie ornamental amazônica Nannostomus beckfordi. Orientador: Carlos Alberto Martins Cordeiro. 2022. 42 f. Trabalho de Curso (Bacharel em Engenharia de Pesca) – Faculdade de Engenharia de Pesca. Instituto de Estudos Costeiros, Campus Universitário de Bragança, Universidade Federal do Pará, Bragança-PA, 2022. Disponível em:https://bdm.ufpa.br:8443/jspui/handle/prefix/4283. Acesso em:.https://bdm.ufpa.br/handle/prefix/4283This study aimed to isolate and select in vitro bacteria with probiotic potential for the Amazon ornamental fish Nannostomus beckfordi. For isolate, twelve fish underwent surgery procedure to remove their intestinal tract, macerate and then inoculate in the plate petri containing de Man Rugosa Sharped Agar (MRS). After bacterial growth (48 hours at 35ºC), selected strains were inoculated in MRS broth and submitted to resistance test with NaCl (0.5, 1.0, 1.5, 2.0, 2.5 and 3.0%), pH (4, 5, 6, 8 and 9) and bile salts (5% w/v). Inhibition test against pathogenic bacteria Aeromonas hydrophila, Pseudomonas aeroginosa, Streptococcus agalactiae and Aeromonas Jandaei was also performed. Within the isolated strains group (23 strains), only six (S1, S2, S3, S4, S5 and S6) showed probiotic potential. Strains S1 and S6 showed the greater resistance for NaCl (0.5% and 1%) and pH (5 and 6), but only S1 obtained better results to resist the bile salts. Even against pathogenic bacteria, the S1 showed the best results with inhibition halos greater than 9 mm. In the end, this bacterial strain (S1) was identified as Enterococcus faecium 11037CHB. Thus, this is the first report regarding isolated autochthonous bacterium E. faecium with probiotic potential of N. beckfordi.Acesso Abertopeixe ornamental amazônicoprobiótico autóctoneensaio in vitroAmazon ornamental fishautochthonous probioticin vitro assayCNPQ::CIENCIAS AGRARIAS::RECURSOS PESQUEIROS E ENGENHARIA DE PESCATrabalho de Curso - Graduação - Monografia