2024-01-252024-01-252022-08-30CUNHA, Giovane Pinheiro. Proteoma diferencial do soro de Canis lupus familiaris acometidos por cinomose canina. Orientadora: Juliana Silva Cassoli. 2022. 58 f. Trabalho de Curso (Bacharelado em Biotecnologia) – Faculdade de Biotecnologia, Universidade Federal do Pará, Belém, 2024. Disponível em: https://bdm.ufpa.br/jspui/handle/prefix/6573. Acesso em:.https://bdm.ufpa.br/jspui/handle/prefix/6573Canine distemper is a multisystemic infection that affects dogs (canis lupus familiares) andsome other species of the carnivorous order and has a high mortality and lethality rate among canids. The diagnosis is based on the animal's clinical history, physical evaluation, and laboratory testing. Proteomics is a tool that has been used to help in the diagnosis of infectious diseases by the identification and tracking of biomarkers produced by the organism in response to infection, or by the detection of proteins of the infectious agent present in the blood of an infected patient. The objective of this work was to describe the differential proteome of dogs infected with Canine Distemper Virus in search of possible protein biomarkers produced by the animals in response to the disease and to detect the presence of viral proteins. Samples were provided by the Biomolecular Technology Laboratory of UFPA (LTB) and analyzed by RT- PCR for confirmation of such infection. Subsequently, the samples were prepared for proteomic analyses in which the proteins present in the serum underwent the processes of reduction, alkylation, digestion, and desalting, generating tryptic peptides. The proteomic analyses were performed using liquid chromatography coupled to a nanoscale mass spectrometry platform (nanoLC-MS/MS). Label-free protein identification and quantification as well as statistical analyses were performed using dedicated software. In addition to it, systems biology analyses were performed using freely available computational tools. The differentially expressed proteins showed correlation with biological processes of the inflammatory response and innate immune response. Thus, by means of descriptive and differential proteomics from the serum of infected dogs it was possible to point out proteins that may contribute to the diagnosis and/or prognosis of the disease.Acesso AbertoCinomose caninaVírus da cinomose caninaProteômicaBiomarcadoresCães - doençasCanis lupus familiarisCNPQ::OUTROS::BIOMEDICINATrabalho de Curso - Graduação - Monografia