2022-11-032022-11-032009SILVA, Carla Elvira Araújo da; LAMARÃO, Maria Fernanda Vita. Estabelecimento de modelo experimental de imunossupressão com ciclofosfamida em primatas não humanos. Orientador: Paulo Pimentel de Assumpção. 2009. 43 f. Trabalho de Curso (Bacharelado em Medicina) - Faculdade de Medicina, Instituto de Ciências da Saúde, Universidade Federal do Pará, Belém, 2009. Disponível em: https://bdm.ufpa.br:8443/jspui/handle/prefix/4629. Acesso em:.https://bdm.ufpa.br/handle/prefix/4629Introduction: Immunosuppressant experimental models have been being established for a few years allowing the development of immune response measurement techniques, the evaluation of new drugs which fights against immune depression and also the study of pathological conditions that are potentialized by immune depression, such as cancer. These are essential tools that allow many future researches, mainly those which involve experimental carcinogenesis. Objective: Establishment of an immunosuppressant experimental model with Cyclophosphamide in non human primates (Cebus apella specie) and evaluate the genotoxicity induced by that drug. Methodology: There were two fases: in the first one, on day zero, blood samples were collected from four healthy monkeys for biochemistry and hematological analysis, which were the negative control of this study. Next, it was injected a single dose of Cyclophosphamide (50mg/kg), e.v., in each animal. There were performed frequent leucograms to determine the period of immune function recovery and then, to inject the second and same dose of Cyclophosphamide, beginning the second fase. To determine the possible genotoxicity induced by Cyclophosphamide in the DNA of those monkeys the Micronuclei Test was performed. Results: The first fase lasted 20 days, from day zero. Between the fourth and the tenth day after the Cyclophosphamide injection the leucocytes levels were lower (6,66±1,40). There was no death caused by intoxication. In the second fase it was injected another dose of Cyclophosphamide passed twenty days from the first one. Two days after that, the leucocytes levels were the lowest ones (3,80±1,44) and this condition remained with no alteration during the next nineteen days. Along this time two monkeys died for intoxication. The Cebus apella’s lymphocytes presented a significant increase in the relation Micronuclei/1000 cells after treatment (P<0.05), however the Micronuclei frequency in the second injection of Cyclophosphamide wasn’t significant. The majority of lymphocytes showed only one Micronucleus per double nucleated cell. Conclusion: Even the beginning of the immunosuppressant status established in the second phase has begun earlier than in the first phase and lasted longer, both immune recovery were complete. As a consequence of the cytotoxic effect of Cyclophosphamide two monkeys from those four of the beginning of this study died. After the treatment the primate’s peripheral blood lymphocytes presented a significant increase in the relation Micronuclei/1000 cells comparing this relation with the control cells. The Cebus apella’s cellular repair system was able to fix and/or eliminate part of the genetic damaged cells, based on the fact that a discreet enhance of the Micronuclei frequency occurred when comparing the second injection of the drug with the first one. The 50mg/kg Cyclophosphamide dose corresponds to the DL50 concentration, considering that 50% of the animals died during this experiment. Until the development of this proposal, that data was unknown in the literature.Acesso AbertoCiclofosfamidaImunossupressãoCebus apellaCyclophosphamideImmunosupressionCebus apellaCNPQ::CIENCIAS DA SAUDE::MEDICINATrabalho de Curso - Graduação - Monografia